1- greiner 3 drops
2- temperature?
3- which screen?
- Dec 2015: Valtteri produced protein: found B2 hit from PCT:
- PCT user guide: https://hamptonresearch.com/documents/product/hr008506_2-140_142_user_guide.pdf
- (From https://hamptonresearch.com/product_detail.aspx?cid=1&sid=29&pid=10 )
- Dec 2016: Anil + Valtteri screened and found hits in JCSG D6 and Morpheus C(1?). Both similar to the PCT hit.
ACTION PLAN for monday 23-10
- 6 aliquots left frozen (use the leftover ones in fridge to test the protocol before burning the fresh aliquots)
- room temperature
- do PCT like Jo advised on greiner 3 well plates
- dispense 0.5ul each PCT solution in each subwell
- dispense 0.5ul protein at 13mg/ml, 10 mg/ml, 8mg/ml in each PCT solution
- wait some 10 minutes
- check in microscope
- 2x JCSG trays at room temp first
- (Lauro used 4degC only after other things failed)
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