20171030 weekly catch up

20171030

• Barbora set JCSG plate and got some 15 hit:
• To cluster them and understand if there are any trends in conditions that show crystalline precipitate (salt conditions vs PEG conditions vs alcohol conditions etc).
• Not to optimise with this protein batch.
• Next protein batch (20 x 20ul aliquotss @ ~13mg/ml) to be ready on Friday 03-11, then to do PCT test for 2,4,8,10,13 mg/ml and use that as reference for what crystallisation condition to use Barbora points out (wisely) that concentrating more could result in full protein crashout given concentrator requires 200ul starting material. 
• Jose's note to self: how does the PCT translate to John William's suggestion to get crashout    vs OD scheme.

20171020 Catch up notes

jose, jo, barbora

1- greiner  3 drops
2- temperature?
3- which screen?

• Dec 2015: Valtteri produced protein: found B2 hit from PCT:
• PCT user guide: https://hamptonresearch.com/documents/product/hr008506_2-140_142_user_guide.pdf
• (From https://hamptonresearch.com/product_detail.aspx?cid=1&sid=29&pid=10 )
•  Dec 2016: Anil + Valtteri screened and found hits in JCSG D6 and Morpheus C(1?). Both similar to the PCT hit.

ACTION PLAN for monday 23-10

• 6 aliquots left frozen (use the leftover ones in fridge to test the protocol before burning the fresh aliquots)
• room temperature
• do PCT like Jo advised on greiner 3 well plates
• dispense 0.5ul each PCT solution in each subwell
• dispense 0.5ul protein at 13mg/ml, 10 mg/ml, 8mg/ml in each PCT solution
• wait some 10 minutes
• check in microscope
• 2x JCSG trays at room temp first
• (Lauro used 4degC only after other things failed)